Center for Global Health Faculty

Associate Director, JHH Medical Mycobacteriology

Academic Degrees

Departmental Address

Contact Information

Email:

nparrish+jhmi.edu

Phone:

410-955-5077

Fax:

410-614-8087

Research and Professional Experience

Tuberculosis (TB) is one of the oldest diseases known to man. Yet despite decades of medical advances, TB remains a major public health menace worldwide. More than 9 million new cases of tuberculosis (TB) are reported each year resulting in 2 million deaths. Even more alarming has been the emergence of multi-drug resistant (MDR) and extensively-drug resistant (XDR) MTB strains. My research focus is twofold, 1) discovery of novel compounds with potent antimycobacterial activity and 2) development of more rapid diagnostics for the detection of drug resistance. A variety of methods lead these approaches including, proteomics, protein and lipid biochemistry as well as current molecular techniques.

Keywords

Tuberculosis, Diagnostics, Drug Development

Honors and Awards

Otis and Calista Causey Honorary Fellowship

President, Graduate Student Association, Molecular Microbiology and Immunology

ARCS Scholar (Achievement Rewards for College Scientists)

Delta Omega Research Award

Member - Delta Omega Honorary Public Health Society

Excellence In Basic Research Award, Department of Pathology, The Johns Hopkins University School of Medicine

Becton Dickinson Centennial Fellowship in Clinical Microbiology

Selected Publications

1. Hendry, C., Dionne, K., Hedgepeth, A., Carroll, K., and Parrish, N. 2009. Evaluation of a Rapid, Fluorescent Stain for the Detection of Mycobacteria in Clinical Specimens. J. Clin. Microbiol. 47(4): 1206-1208.

2. Parrish, N., Dionne, K., Sweeney, A., Hedgepeth, A., and Carroll, K. 2009. Differences in Time to Detection and Recovery of Mycobacterium species between the MGIT 960 and the BacT/ALERT MB Automated Culture Systems. Diagn. Microbiol. Infect. Dis. 63(3): 342-345.

3. Parrish NM, Radcliff RP, Brey BJ, Anderson JL, Clark DL Jr, Koziczkowski JJ, Ko CG, Goldberg ND, Brinker DA, Carlson RA, Dick JD, Ellingson JL. 2009. Absence of Mycobacterium avium subsp. paratuberculosis in Crohn's patients. Inflamm Bowel Dis. (Epub ahead of print).

4. Parrish,N., and Carroll, K. 2008. Importance of improved TB diagnostics in addressing the extensively drug-resistant TB crisis. Future Microbiol. 3: 405-413.

5. Parrish, N., Osterhout, G., Dionne, K., Sweeney, A., Kwiatkowski, N., Carroll, K., Jost, K.C., Jr., and Dick., J. 2007. A Rapid, Standardized, Susceptibility Method for Mycobacterium tuberculosis using Mycolic Acid Analysis. J Clin. Micro. 45: 3915-3920.

6. Dionne, K., Sweeney, A., Hedgepeth, A., Carroll, K., and Parrish, N. 2005. Methods for reducing bacterial contamination in the BacT/Alert Mycobacterial Culture Detection System. J. Clin. Micro. 43(5): 2523-5.

7. Parrish, N.M., C. Ko, J. Dick, P. Jones, J. Ellingson. 2004. Growth, congo red agar colony morphotypes and antibiotic susceptibility testing of M.avium subspecies paratuberculosis. Clinical Medicine and Research. 2: 107-114.

8. Parrish, N.M., C.A. Townsend, C.G. Ko, and J.D. Dick. 2004. Effect of n-Octanesulfonylacetamide (OSA) on ATP and protein expression in Mycobacterium bovis BCG. J. Antimicrob. Chemother. 54(4): 722-729.

9. Ginsburg A.S., Hooper, N., Parrish, N., Dooley, K.E., Dorman, S.E., Booth, J., Diener-West, M., Merz, W.G., Bishai, W.R., Sterling, T.R. 2003. Fluoroquinolone resistance in patients with newly diagnosed tuberculosis. Clin Infect Dis. 7(11): 1448-52.

10. D.W. Dowdy, A. Maters, N. Parrish, C. Beyrer, and S. Dorman. 2003. Cost-Effectiveness analysis of the Gen-probe amplified Mycobacterium tuberculosis direct test as used routinely on smear-positive respiratory specimens. J. Clin. Micro. 41: 948-953.

11. Parrish, N.M, C. Townsend, P. Jones, T. Houston, and J. Dick. 2001. In vitro activity of a novel antimycobacterial compound, N-octanesulfonylacetamide, and its effects on lipid and mycolic acid synthesis. Antimicrob. Agents Chemother. 45:1143-1150.

12. Jones, P.B., N.M. Parrish, T.A. Houston, A. Stapon, N.P. Bansal, J.D. Dick, and C.A.Townsend. 2000. A new class of antituberculosis agents. J. Med. Chem. 43(17): 3304-14.

13. Dannenberg, A.M., Jr., W.R. Bishai, N.M. Parrish, R. Ruiz, W. Johnson, B.C. Zook, J.W. Boles, and L.M. Pitt. 2000. The efficacies of BCG and Vole Bacillus (Mycobacterium microti), vaccines in preventing clinically apparent pulmonary tuberculosis in rabbits: a preliminary report. Vaccine. 19: 796-800.

14. Parrish, N.M., F.P. Kuhajda, H.S. Heine, W.R. Bishai, and J.D. Dick. 1999. Antimycobacterial activity of cerulenin and its effects on lipid biosynthesis. Journal of Antimicrobial Chemotherapy. 43: 219-226.

15. Bishai, W., A. Dannenberg, Jr., N. Parrish, R. Ruiz, P. Chen, B. Zook, W. Johnson, J. Boles, and M. Pitt. 1999. Virulence of Mycobacterium tuberculosis CDC 1551 and H37Rv in rabbits evaluated by Lurie’s Pulmonary tubercle count method. Infect. Immun. 67(9): 4931-4.

16. Parrish, N.M., J. D. Dick and W.R. Bishai. 1998. Mechanisms of latency in Mycobacterium tuberculosis. Trends in Microbiology. 6: 107-112.